If image ='s....

how do i do this

If picture.Image.fromfile = application.StartupPath "grass.bmp" Then
'Do my stuff here
end if

how would i get the file path of the image in a picturebox?

Do you load the Image in the PictureBox using the path?

Well, the image that is in a PictureBox doesn't have to have an associated file on the user's computer so unless you loaded the file and saved the path when doing so then you are out of luck.